CELL AND NEOPLASM BIOLOGY - Teaching aids

Lecture:
Lecture 5 - Cell signaling. Cancer pathways
Lecture 4 - Regulation of gene expression and its implication for human diseases

Lab 5 - 18 & 19.03.2020
Dear Students,
Please study the following materials:
1. Textbook chapter according to the Syllabus
2. Lecture 5 presentation
3. Evaluation – worksheet Lab5 and Review PT1 presentation. Please answer all of the questions.
I strongly encourage you to study systematically.

Joanna Kozak, MSc., Ph.D

Lab 5 - Review 1 - presentation
Lab 5 - Review 1 - worksheet

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Lab 4
Dear Students,
I prepared for you all essential materials for lab 4 (cell and neoplasm course):
1. Lab presentation
2. Two videos (links are on last slide in lab presentation)
3. Worksheet with evaluation questions.
Please, go through all materials and do seven questions at the end of worksheet.

Joanna Kozak, MSc., Ph.D

Lab 4 - Techniques for gene expression research quantitative PCR - presentation
Lab 4 - Techniques for gene expression research quantitative PCR - worksheet

Recommended material for the labs:
Lab 1 - AN3CA
Lab 1 - Protocol cell culture
Lab 2 - ATCC - Guide to subculturing cell line monolayers
Lab 3 - mirVana PRIS Kit
Lab 4 - Gene expression analysis
Lab 4 - High capacity cDNA reverse transcription kits
Lab 4 - Real-time qPCR data analysis
Lab 4 - TaqMan® Small RNA Assays
Lab 5 - mirVana PRIS Kit
Lab 6 - SDS PAGE cytophysiology
Lab 7 - Western Blotting cytophysiology
Lab 8 - Apo Tox Glo Triplex Assay
Lab 8 - CytoSelect™ 96-Well Anoikis Assay
Lab 9 - FITC Annexin VDead Cell Apoptosis Kit with FITC annexin V and PI, for flow cytometry